Insertion junction: LMJ.SG0182.013696_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.013696
Chromosome:chromosome 6
Location:7505898
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g300500 ADCY1,CYA18 Adenylyl cyclase; (1 of 1) PTHR11347//PTHR11347:SF132 - CYCLIC NUCLEOTIDE PHOSPHODIESTERASE // PDE1C, ISOFORM E intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTTGCCTTCCTCATGCAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:695
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCAAACACCCACATCACT
Suggested primer 2:GCTTACGAGACCGAGGTGAG