Insertion junction: LMJ.SG0182.013742_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.013742
Chromosome:chromosome 12
Location:8390572
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g548950 LHCBM7 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGTCAGTATCGAGTTCGCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:699
LEAP-Seq percent confirming:98.6301
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TGACATTCTGGCACTTCTGC
Suggested primer 2:GCCTGTGAAAAGAGGCTCAC