| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.013859 |
| Chromosome: | chromosome 10 |
| Location: | 3432440 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444600 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCCAACGGGCCAGGCCCC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1509 |
| LEAP-Seq percent confirming: | 99.7214 |
| LEAP-Seq n confirming: | 4653 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGTTGTCCCCGGTAGTA |
| Suggested primer 2: | GATCGCTCCGAGTCTACCTG |