Insertion junction: LMJ.SG0182.013908_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.013908
Chromosome:chromosome 12
Location:5835859
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g533600 DESI6,DeSI-6 (1 of 6) PF05903 - PPPDE putative peptidase domain (Peptidase_C97); DeSI-type SUMO protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAGCCCTTGACAGAGTCTG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:546
LEAP-Seq percent confirming:98.4733
LEAP-Seq n confirming:129
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTTAAGGCTGTCGCTCA
Suggested primer 2:CAGAACCAAGGCCGTGTAAG