Insertion cassette: | pMJ013b |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.SG0182.013908 |
Chromosome: | chromosome 12 |
Location: | 5835859 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g533600 | DESI6,DeSI-6 | (1 of 6) PF05903 - PPPDE putative peptidase domain (Peptidase_C97); DeSI-type SUMO protease | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCCCTTGACAGAGTCTG |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 546 |
LEAP-Seq percent confirming: | 98.4733 |
LEAP-Seq n confirming: | 129 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATGTTAAGGCTGTCGCTCA |
Suggested primer 2: | CAGAACCAAGGCCGTGTAAG |