Insertion junction: LMJ.SG0182.014028_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.014028
Chromosome:chromosome 6
Location:5753686
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g287176 CPLD67 dihydrolipoyllysine-residue acetyltransferase; (1 of 5) PF01713 - Smr domain (Smr) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTGCACAAGCTGTCCATCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:681
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACACCCACCAAAACAAT
Suggested primer 2:TGTAGCTGCTTGGTCTGTGG