Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.014079 |
Chromosome: | chromosome_4 |
Location: | 2019387 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g218900 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACGCTACAGGTTTGTCGGCAT |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1455 |
LEAP-Seq percent confirming: | 96.3986 |
LEAP-Seq n confirming: | 803 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATGGCAACCAAGTTCTTT |
Suggested primer 2: | TTCGATTAGTGCAGGTGCTG |