Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.014236 |
Chromosome: | chromosome 4 |
Location: | 3807553 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGCACTGGAGTCGGAG |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 915 |
LEAP-Seq percent confirming: | 94.7917 |
LEAP-Seq n confirming: | 1001 |
LEAP-Seq n nonconfirming: | 55 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTTGTTGTGGTTGCTGCT |
Suggested primer 2: | GTCTTTGCGCTTTGTTCTCC |