Insertion junction: LMJ.SG0182.014454_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.014454
Chromosome:chromosome 4
Location:2849443
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre04.g224300 CGL84 (1 of 22) PTHR10994 - RETICULON; Conserved in the Green Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAAATGTACTCTGATCCAT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1209
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:585
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCACATAAAGCAGGAGCACA
Suggested primer 2:CGTCATTTCCAACCAGACCT