Insertion junction: LMJ.SG0182.014455_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.014455
Chromosome:chromosome 6
Location:3290123
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g277100 (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAACCAGCGCACTCAACAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:650
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:145
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AAATTCACCCGCTTGCTCTA
Suggested primer 2:TGGGTTGCATACACAAGCAT