Insertion junction: LMJ.SG0182.015103_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.015103
Chromosome:chromosome 16
Location:6010944
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre16.g676450 CPLD39 Conserved in the Plant Lineage and Diatoms intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGTAGTCGGATGAGAGGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:972
LEAP-Seq percent confirming:98.3607
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCGGTAACGATTGCACTT
Suggested primer 2:CAACGCCACCTCACCTTATT