| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.015259 |
| Chromosome: | chromosome 12 |
| Location: | 7114268 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559250 | POC14,FTT1 | (1 of 2) K06630 - 14-3-3 protein epsilon (YWHAE); Proteome of centriole protein 14 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGGGCGCGCAAAGGAAT |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1406 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 791 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAATGGGCACCAGTACGTC |
| Suggested primer 2: | ACACACCTTGCATTTAGCCC |