Insertion junction: LMJ.SG0182.015389_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.015389
Chromosome:chromosome 5
Location:2703614
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre05.g236700 (1 of 781) IPR000104 - Antifreeze protein, type I MULTIPLE_SPLICE_VARIANTS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCGCAGGCCAACCTGGAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1035
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTACACGATACCCAAGCG
Suggested primer 2:GTAAACACACGCACCGTGAC