Insertion junction: LMJ.SG0182.015559_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.015559
Chromosome:chromosome 12
Location:1941653
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g510600 STK11,STPK11 Serine/threonine protein kinase; (1 of 1) 2.7.11.1//2.7.11.21 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Polo kinase / Polo-like kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGAGAAGTCCCTCTGAAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1206
LEAP-Seq percent confirming:98.513
LEAP-Seq n confirming:265
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTCGGTCTCACTTAGGC
Suggested primer 2:TGTTCAACACACCCGGTCTA