Insertion junction: LMJ.SG0182.015559_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.015559
Chromosome:chromosome 12
Location:1941662
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g510600 STK11,STPK11 Serine/threonine protein kinase; (1 of 1) 2.7.11.1//2.7.11.21 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Polo kinase / Polo-like kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCGCCCTCCGTCAGGAT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:616
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTCGGTCTCACTTAGGC
Suggested primer 2:TGTTCAACACACCCGGTCTA