Insertion junction: LMJ.SG0182.015645_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.015645
Chromosome:chromosome 5
Location:1645229
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g5283 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGGCCGCCGCCGACACA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:665
LEAP-Seq percent confirming:97.6744
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGGAGGAGAAAGAGGAAG
Suggested primer 2:TAGGTCAGCCAAGCACACAG