Insertion junction: LMJ.SG0182.015793_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.015793
Chromosome:chromosome 12
Location:5528280
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g531200 FOX2 Multicopper ferroxidase; (1 of 2) K14735 - hephaestin (HEPH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGCCAAATGTGATGGGA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1081
LEAP-Seq percent confirming:90.9657
LEAP-Seq n confirming:292
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGCTCTCTATCACGGAC
Suggested primer 2:CGTGACTTTCAGAACTGCCA