Insertion junction: LMJ.SG0182.015793_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.015793
Chromosome:chromosome 12
Location:5528280
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g531200 FOX2 Multicopper ferroxidase; (1 of 2) K14735 - hephaestin (HEPH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGGCCATTCTGGACCACAG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:747
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:126
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGCTCTCTATCACGGAC
Suggested primer 2:CGTGACTTTCAGAACTGCCA