| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.015831 |
| Chromosome: | chromosome 1 |
| Location: | 5763130 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041150 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGACGCCGCATCGATTTC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1385 |
| LEAP-Seq percent confirming: | 93.858 |
| LEAP-Seq n confirming: | 489 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACGTGGATCACGCCTTT |
| Suggested primer 2: | AGTTGTTGTGGGAGGAGGTG |