Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.016119 |
Chromosome: | chromosome 7 |
Location: | 5598596 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g351850 | NAT21 | (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGTGCGTCAAGTAGCA |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 849 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 134 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAGGAACGGAAGATTCTCA |
Suggested primer 2: | TCCAGCAGTCGTGAGAAATG |