Insertion junction: LMJ.SG0182.016129_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.016129
Chromosome:chromosome 16
Location:3707255
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre16.g688900 AMC1 Assembly of mitochondrial complex I 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGGGGAAGCCTCATCGCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:780
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:95
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGTTGTGTGGTCCTTTGG
Suggested primer 2:CACCTCCTGGCCATAACTGT