Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.016143 |
Chromosome: | chromosome 3 |
Location: | 6585307 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g196150 | uL5m,MRPL5 | (1 of 3) IPR022803 - Ribosomal protein L5 domain; Mitochondrial ribosomal protein L5 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGGGCCTGGTAGGTGCA |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1378 |
LEAP-Seq percent confirming: | 99.7605 |
LEAP-Seq n confirming: | 833 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTACCCGGACTTCGAACAAA |
Suggested primer 2: | GTTTCGTTCGCAATGGATTT |