Insertion junction: LMJ.SG0182.016217_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.016217
Chromosome:chromosome 1
Location:7374103
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre01.g053000 GPD1,GPDH2,GPD2 (1 of 3) K00006 - glycerol-3-phosphate dehydrogenase (NAD+) (GPD1); Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGTGAGTGGGGTACCCAT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:800
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:231
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGCAGTTGATCAGGTTGA
Suggested primer 2:TACCTTTCGGTTGGGACTTG