Insertion junction: LMJ.SG0182.016384_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.016384
Chromosome:chromosome 17
Location:2496107
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre17.g716100 DNA polymerase lambda CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACAGGCGCCAGTACCTCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:519
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTGTTGTTGAGCTGAC
Suggested primer 2:GGTCATGTTAGGGACGCTGT