Insertion junction: LMJ.SG0182.016489_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.016489
Chromosome:chromosome 5
Location:3066677
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre05.g238935 (1 of 781) IPR000104 - Antifreeze protein, type I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGTCGGCGTCAGCATGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:880
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGCCATAGTCTGTGTACC
Suggested primer 2:GTGCTTCTTCCTCAAGACCG