Insertion junction: LMJ.SG0182.016542_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.016542
Chromosome:chromosome 10
Location:3674323
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre10.g445900 (1 of 1) PF00226//PF14901 - DnaJ domain (DnaJ) // Cleavage inducing molecular chaperone (Jiv90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGATCCAAAGTCGACTGATT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:914
LEAP-Seq percent confirming:97.8887
LEAP-Seq n confirming:510
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGTACAGGGGTTGTTCTA
Suggested primer 2:TGCAGCATCAGGTGTAAAGC