Insertion junction: LMJ.SG0182.016911_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.016911
Chromosome:chromosome 16
Location:5994504
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre16.g676600 (1 of 4) IPR029041 - FAD-linked oxidoreductase-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGACTGAAACCAGAGCTCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:807
LEAP-Seq percent confirming:95.9596
LEAP-Seq n confirming:380
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTGTCGTTCTGGGTCAG
Suggested primer 2:TTTCTACGTGCATTCAAGCG