Insertion cassette: | pMJ013b |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.SG0182.016966 |
Chromosome: | chromosome 8 |
Location: | 5021031 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g386100 | CYP744A5P | MULTIPLE_SPLICE_VARIANTS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTCTGTTTGGATGTCGT |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 690 |
LEAP-Seq percent confirming: | 99.2481 |
LEAP-Seq n confirming: | 132 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAGCACTTGCGTGTGTACG |
Suggested primer 2: | ACGCACAGGATGGACACATA |