Insertion junction: LMJ.SG0182.017019_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.017019
Chromosome:chromosome 6
Location:1231205
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g257800 POL1B,POL1-1 (1 of 2) IPR001098//IPR002298 - DNA-directed DNA polymerase, family A, palm domain // DNA polymerase A; DNA polymerase I, probably mitochondrial 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGACGGCATCCGCCAGGAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1188
LEAP-Seq percent confirming:99.6116
LEAP-Seq n confirming:513
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GATGGGCGTGTGTCAGTATG
Suggested primer 2:CGGTGTGAACATTGGACTTG