Insertion junction: LMJ.SG0182.017096_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.017096
Chromosome:chromosome 8
Location:36144
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre08.g358400 (1 of 4) PF05653 - Magnesium transporter NIPA (Mg_trans_NIPA) MULTIPLE_SPLICE_VARIANTS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCGCGCAGGCAAGGGAGC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1349
LEAP-Seq percent confirming:99.4624
LEAP-Seq n confirming:370
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCAGTACATGTCTCCCA
Suggested primer 2:TGTGTCTTGCTCAAAAACGC