Insertion junction: LMJ.SG0182.017320_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.017320
Chromosome:chromosome 13
Location:3731497
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCCTCGGGGTCTGCAAGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1304
LEAP-Seq percent confirming:82.0513
LEAP-Seq n confirming:224
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTTCTCTCACACCTTGGG
Suggested primer 2:CCGTATACGTGGTGGAGGAC