Insertion junction: LMJ.SG0182.017397_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.017397
Chromosome:chromosome 14
Location:2211837
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre14.g622850 RAD51A,RAD51 (1 of 1) K04482 - DNA repair protein RAD51 (RAD51); RAS associated with diabetes protein 51 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCGCTTCCTAGGTTGACA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1291
LEAP-Seq percent confirming:99.6416
LEAP-Seq n confirming:278
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGTTTAGTTTGGGCTGG
Suggested primer 2:AAGGTAGCACTGTTGGTGGG