| Insertion cassette: | pMJ013b |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.SG0182.017434 |
| Chromosome: | chromosome 2 |
| Location: | 453744 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076400 | VPS25 | Subunit of ESCRT-II complex; (1 of 1) K12189 - ESCRT-II complex subunit VPS25 (VPS25, EAP20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGAACATTGACCTGCAAG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACTGCTCAGCCAGAATC |
| Suggested primer 2: | GTGTGTCCCCACTTGCTTTT |