Insertion junction: LMJ.SG0182.017708_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.017708
Chromosome:chromosome 9
Location:3354996
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g9619 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACAGGCTAACCTCCACACG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1670
LEAP-Seq percent confirming:88.015
LEAP-Seq n confirming:235
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGTGGTGTGGGTCGTAG
Suggested primer 2:TGGGGGTTACATTCATGGTT