Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.017998 |
Chromosome: | chromosome 10 |
Location: | 256637 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
g10383 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGGGACATGCGGCTGAG |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1414 |
LEAP-Seq percent confirming: | 89.0468 |
LEAP-Seq n confirming: | 1504 |
LEAP-Seq n nonconfirming: | 185 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTACAGGCTCTGCGCTCAT |
Suggested primer 2: | GAGTCTGTATCCCGCTCTGC |