Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.SG0182.018065 |
Chromosome: | chromosome 12 |
Location: | 7936979 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g552400 | (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTTTGCTGTGTGCAGGAT |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1032 |
LEAP-Seq percent confirming: | 96.9582 |
LEAP-Seq n confirming: | 765 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCAACAGCGACTCCATGA |
Suggested primer 2: | TCTGCTGTAGCTGACGCACT |