Insertion junction: LMJ.SG0182.018216_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.018216
Chromosome:chromosome 6
Location:823569
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g5720 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCATCCCCATCTCGCTA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:764
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTCATACGTCAGCAAGTCGC
Suggested primer 2:AAGAGCCATAGCGCAGGTAA