Insertion junction: LMJ.SG0182.018403_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.018403
Chromosome:chromosome 10
Location:4794485
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre10.g453807 CTPB1 (1 of 2) PTHR32060:SF5 - PEPTIDASE S41 FAMILY PROTEIN; C-terminal peptidase B MULTIPLE_SPLICE_VARIANTS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACAAACAGCGCTGAGCCG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:99.6855
LEAP-Seq n confirming:317
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCCACGTCAACAAACAGCTA
Suggested primer 2:ATACGCTGTCTTTGCGCTTT