| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.018494 |
| Chromosome: | chromosome 7 |
| Location: | 1079609 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319800 | (1 of 1) IPR000104//IPR009148 - Antifreeze protein, type I // Streptococcal non-M secreted SibA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTACGGCAGGTGCTGGTT |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1338 |
| LEAP-Seq percent confirming: | 95.3321 |
| LEAP-Seq n confirming: | 1062 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACATGTTCGGCAATGGAA |
| Suggested primer 2: | GCGTCGGTTGATAGTTGGTT |