Insertion junction: LMJ.SG0182.018505_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.018505
Chromosome:chromosome 9
Location:1142144
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre09.g400150 (1 of 2) PF13415//PF13418 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGTGCGAGTCCGTCTAGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:711
LEAP-Seq percent confirming:85.0575
LEAP-Seq n confirming:444
LEAP-Seq n nonconfirming:78
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGAGCAGTCGTGCCATAC
Suggested primer 2:CAAAACGACGTTCAGTCGAA