Insertion junction: LMJ.SG0182.018520_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.018520
Chromosome:chromosome 6
Location:4146021
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g6368 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTAAAGAAGTTGGGGTCG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1090
LEAP-Seq percent confirming:97.5904
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTCCACCAGCTCGTAGTG
Suggested primer 2:GGAATAGAGGGTCGATGCAA