| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.018530 |
| Chromosome: | chromosome 6 |
| Location: | 7307024 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298750 | AOT4 | (1 of 9) PF01490 - Transmembrane amino acid transporter protein (Aa_trans); Amino acid transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATCTACGCCGCCCTAGAGA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1272 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 409 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAAATGCCGACCTTGTC |
| Suggested primer 2: | GACAGGGAACCACTTCGTGT |