Insertion junction: LMJ.SG0182.018605_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.018605
Chromosome:chromosome_16
Location:4951888
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g685450 DHC8 Flagellar inner arm dynein heavy chain, dynein e (monomeric) sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):ATTTCGCGCAGGAACTTGAAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:989
LEAP-Seq percent confirming:99.0654
LEAP-Seq n confirming:212
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGCTGGGAGTGTACCTA
Suggested primer 2:TCATGAGGTTCTCCACCTCC