| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.018730 |
| Chromosome: | chromosome 2 |
| Location: | 3351537 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| g1869 | PHC12 | Cell wall protein pherophorin-C12 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGACTTCCGTTTGGAAC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1040 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTCTGGTCTTGTGTGTA |
| Suggested primer 2: | CGCACCTGCTGATTAACTGA |