Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.SG0182.018797 |
Chromosome: | chromosome 12 |
Location: | 594236 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g492550 | (1 of 781) IPR000104 - Antifreeze protein, type I | MULTIPLE_SPLICE_VARIANTS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCTGGCAGGTACAGCG |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 994 |
LEAP-Seq percent confirming: | 88.7218 |
LEAP-Seq n confirming: | 118 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGGTCCATGATATCCCAAA |
Suggested primer 2: | AGTACACACACAAGCTGCCG |