Insertion junction: LMJ.SG0182.018810_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.018810
Chromosome:chromosome 16
Location:2044734
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre16.g657100 (1 of 781) IPR000104 - Antifreeze protein, type I 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTATCGCCCAAGCGCGCTC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:906
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTTTTCTCATACCTTGC
Suggested primer 2:AAGGGTTTGGATGTTTGCAG