| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.018846 |
| Chromosome: | chromosome 17 |
| Location: | 6080130 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741300 | SAK1 | Regulator of acclimation to singlet oxygen | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGATGCCGGCCCATGCCTG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1194 |
| LEAP-Seq percent confirming: | 94.5652 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGGCTGTGTTGCTACTG |
| Suggested primer 2: | TCCAAAATCTGAAGATGGGC |