Insertion junction: LMJ.SG0182.018945_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.018945
Chromosome:chromosome 12
Location:1690299
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g485750 CGL128 (1 of 1) PTHR13420//PTHR13420:SF5 - UNCHARACTERIZED // UPF0235 PROTEIN; Conserved in the Green Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACATCCTGGACAACGCCGC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1016
LEAP-Seq percent confirming:98.7179
LEAP-Seq n confirming:154
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CTATCAGTTTCCAGCCTCGC
Suggested primer 2:CTTGCTGATGAGGTCGAACA