Insertion junction: LMJ.SG0182.018968_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.018968
Chromosome:scaffold 19
Location:134564
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g18135 MULTIPLE_SPLICE_VARIANTS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTTCCCCCCTCTCCAAACC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:413
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:116
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ATAAGAATCGCAACAACCCG
Suggested primer 2:CGACTGGAAAGTGGAGAAGC