Insertion junction: LMJ.SG0182.019064_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.019064
Chromosome:chromosome 12
Location:3956364
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g516450 CAG1 Gamma carbonic anhydrase 1; (1 of 1) PTHR13061//PTHR13061:SF7 - DYNACTIN SUBUNIT P25 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGCCCTCCTGAGACGTCCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1415
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:458
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCGTTGAAGATGCTGTC
Suggested primer 2:TAGCTAGCTTGTGGCCTGGT