| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.019097 |
| Chromosome: | chromosome 12 |
| Location: | 5606563 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532000 | (1 of 3) PF04359 - Protein of unknown function (DUF493) (DUF493) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTAGCACCTCGAACCAGGC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1284 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAAGCGCGATAAGCATCC |
| Suggested primer 2: | TGAGTCCGGTAATCTTTCGG |